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Both flows (draw and feed) were adjusted at 0.3 L per minute, and sampling was performed 20 min after the beginning of the process

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The studies analyzed consistently highlight adolescence as a critical period of neurobiological maturationparticularly within the prefrontal cortex and limbic regions that regulate cognition, emotion, and decision-makingduring which nicotine exposure may confer heightened vulnerability ( Nicotine acts on nicotinic acetylcholine receptors, affecting neurotransmission and synaptic plasticity