Human CD34 + cells were cultured in vitro using complete medium (CM) consisting of StemSpan II (StemCell Technologies) medium supplemented with 100 ng l 1 human stem cell factor (R&D systems, 255-SC/CF), 100 ng l 1 human thrombopoietin (R&D systems, 288-TP/CF) and 100 ng l 1 human FLT-3 ligand (R&D systems, 308-FK/CF)
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active smoking exhibited a positive association with the eGFR cr , a negative association with the eGFR cys and a non-significant association with the eGFR average
Colchicine inhibited senescence in endothelial cells exposed to smoke condensate Oxidative stress and oxidative stress-induced DNA damage can cause cellular senescence
