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Afterwards, the Pyrosequencing PCR kit (Qiagen) was used to amplify the mtDNA regions
Cochrane Database of Systematic Reviews, 4 , CD013308 Mitchell JT, Howard AL, Belendiuk KA, Kennedy TM, Stehli A, Swanson JM,
When looking at perceived addictiveness relative to smoking tobacco, the use of e-cigarettes within 5 minutes of waking and daily vaping were associated with a higher likelihood of viewing e-cigarettes as equally or more addictive compared to cigarettes
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