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The thermal cycling steps were: 50 C for 2 min, 95 C for 10 min, and 40 cycles of 95 C for 15 s and 60 C for 1 min, and qPCR was performed on a Step-One-Plus real-time PCR machine (Applied Biosystems) using the StepOne Software v2.3

DOI: 10.1016/j.oraloncology.2019.104550

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One consequence of the 1998 Master Settlement Agreement (MSA) was a drastic increase in the price of a pack of cigarettes 41