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Park HY, Kang D, Shin SH, Choi H, Jang SH, Lee CH, Kim H, Kwon OJ, Rhee CK, Cho J
After systematic optimization of the entire workflow, where we varied circularized gDNA exonuclease treatments, base-editing reaction conditions and end-repair protocols, we identified an optimized protocol (Supplementary Note 1 and Supplementary Protocol) that consistently detected all 54 previously validated ABE8e-NRCH: HBB S -gRNA off-target sites, which we called CHANGE-seq-BE (Extended Data Fig
Nevertheless, the total marketing expenditure remained unaffected
Pregnant women now have another reason to quit smoking - a new analysis links it to differences in their babies' DNA that mirror alterations in adult smokers and suggest how smoking might contribute to certain birth defects
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