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Sources Centers for Disease Control and Prevention (CDC) Nicotine and Tobacco Use National Institute on Drug Abuse (NIDA) Nicotine Research Reports U.S

Removing nicotine removes the addiction driver

Western blot Cells were lysed with lysis buffer (RIPA buffer from Thermo Scientific, 89900, 1X Halt protease inhibitor cocktail from Thermo Scientific, 78430, and 1X Halt phosphatase inhibitor cocktail from Thermo Scientific, 78426)

This may be helpful to choose the range of exposure doses in in vitro assays, but also for the interpretation of the in vitro test results to the in vivo situation

However, this can vary based on factors like tobacco type, cigarette design, and individual smoking habits