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Isolated RNA was normalized to 3.5 ng input/sample for automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit, using 12 cycles of amplification

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Therefore, the changes in the size and composition of longitudinal bundles in smoking asthmatics may further aggravate airway remodeling by affecting the mechanical properties of the airway or the folding behavior of the airway mucosa 45,83