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The forward primer for ITS gene PCR amplification is ITS1 (sequence: CCGTAGGTGAACCTGCGG), and the reverse primer is ITS4 (sequence: TCCTCCGCTTATTGATATGC), with an amplification length of approximately 500 bp

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Italy E-Cigarette Market Revenues, By Device Type, 2018 & 2025F ($ Million) Figure 95

Cravings Dizziness Insomnia Tiredness Irritability Cough and sputum Constipation Taste for sweet food and cravings Cravings Even if they last only a few minutes, cravings are probably the most disturbing withdrawal symptom because they can be very intense, especially at the beginning

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