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It is a symbolic indication of the achievement of marital status and represents the responsibility that is peculiar to a married woman, in contrast to the carefree life-style of an unattached maiden

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The Thermocycler (C1000 Thermal cycler, Bio-Rad, USA) was used following this program: Initial denaturation at 95 C for 3 min followed by 35 cycles of denaturation at 95 C for 30 s

But canker sores often are