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A Cox proportional hazard regression model was used to evaluate the effects of in utero tobacco exposure, age of smoking initiation, and environmental pollution on COPD incidence

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One day after implantation, mice were divided into two groups (8 mice/group), the first group was administered with nicotine (2 mg/kg, every other day) while second group received nicotine plus natural compound Salidroside (20 mg/kg, every other day, Sigma-Aldrich) through intraperitoneal injection till the endpoint