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The 2xStrep-tag II-6xHis-Xpress epitope-linked RfA-cassette was amplified using as template pTK249, a pEXP1-Dest (Invtirogen) derivative encoding a 2xStrep-tag II-6xHis-Xpress epitope-linked RfA-cassette (see Supplementary Methods) and primers Strep-5-PciNdePac and Exp1-Dest-3-PspAvr

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