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Quantitative real-time PCR Total RNA was isolated using the RNAeasy Animal RNA Isolation Kit (Beyotime, R0026), and cDNA was synthesized using the HiScript IV RT SuperMix for qPCR (Vazyme, R423-01)

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References: Also read: Breaking The Silence On Oral Cancer: Why Routine Dental Screenings Are Vital For Tobacco Users World No Tobacco Day 2026: Exploring The Impact Of Smoking On Bone Health India's Tobacco Quitline Receives Nearly 1 Crore Calls In Last Decade, Helps 2.32 Lakh Users Quit Rajasthan Gets WHO Award For Effective Tobacco Control Programme