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Thereafter, ME mucosal thickness was measured from three selected areas to obtain an overall average thickness, including one area where an imaginary line was drawn from the cochlea to the opposite bulla wall, and two areas where a line perpendicular to the imaginary line was drawn to meet the bulla walls by using a Leica Aperio CS2 with Aperio ImageScope and an image analysis system (Leica Microsystems Imaging Solutions Ltd., Cambridge, UK) (Suppl
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Low signal clusters characterized by a high ribosomal gene signature and/or low UMI count and cell multiplets were identified by cluster analysis and excluded from further analysis
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