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Discussion Cigarette smoke extracts have been widely used for various in vitro and in vivo toxicity studies on smoking, and the results obtained closely match those obtained using exhaled mainstream smoke under controlled conditions [59]

Finally, the equipment used in this study, although considered new-generation, is already outdated

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Smoking might indirectly influence estrogen levels due to its impact on overall hormone regulation, but nicotine itself isnt known to increase estrogen directly