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Dedifferentiated RPE had globally decreased chromatin accessibility and expression of genes linked to hallmarks of aging. Notably, young, dedifferentiated RPE also exhibited a compensatory upregulation of hallmarks of aging-related genes including mitochondrial function and proteostasis while aged dedifferentiated RPE did not, which decreased their survival following CSC treatment, as experimentally verified with TUNEL labeling

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Considerably smaller grey matter quantities, as well as lower grey tissue thickness, were noted in the frontal regions, the temporal lobe and also the occipital wattle in cigarette smokers compared to their counterparts