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Spliffs: Paper wraps containing a mixture of cannabis and tobacco

Extracellular flux assaymitochondrial stress test Cells were plated at a density of 7500 cells/well in XF96 micro-plates (Agilent Technologies, Santa Clara, CA, USA) in DMEM/F12 supplemented with 10% FBS 48 h prior to the assay and cultivated in a humified incubator at 37 C with 5% CO 2

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Variables worth assessing include: Amount smoked Degree of dependence (e.g., cigarettes per day, time to first cigarette) Patterns of smoking Reasons for smoking Reasons for quitting Experience with quitting: what worked and what didn't Previous relapse triggers Other addictions, medical problems, psychiatric problems, medications Current interest in quitting Current confidence in being able to quit

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