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For inputs, a fraction of this worm suspension was briefly spun down and Buffer A was replaced with 3 volumes of lysis buffer (25 mM HEPES-KOH pH 7.5, 150 mM KCl, 0.5% NP-40, 0.1% Triton X-100, protease and phosphatase inhibitors) and crushed with a metal douncer on ice with 100 strokes

Cigarette advertising in Mumbai, India: targeting different socioeconomic groups, women, and youth